Alternative location methods for absolute positioning in areas where no GNSS position determination is possible due to obstruction of the satellite signals are needed in mobile positioning. Active RFID (Radio Frequency Identification) can be used also for position determination, although the system was not only developed for positioning and tracking but mainly for identification of objects. Using RFID in positioning, different approaches can be distinguished, i.e., cell-based positioning if the RFID tags are installed at active landmarks (i.e., known locations) in the surroundings, trilateration if ranges to the RFID tags are deducted from received signal strength (RSS in RFID terms) values and location fingerprinting where the measured signal power levels are used directly to obtain a position fix. Using Cell of Origin (CoO) the achievable positioning accuracy depends on the size of the cell and is therefore usually several metres up to 10’s of metres using long range RFID equipment. Higher positioning accuracies can be obtained using trilateration and fingerprinting. In this paper the use of trilateration is investigated.